High-performance Hydrophobic Interaction Chromatog-raphy of Proteins on TSKgel Phenyl-5PW Preparative Column

TSKgel Pheny1-5PW preparative column of 200 × 55 mm I.D.was evaluated with respect to resolution,sample loading capacity and application to the purification of enzymes.The preparative column provided similar separations as analytical column (75×7.5 mm I.D) and 150 ×21.5mm　I.D.preparative column.The sample loading capacity was 200-1000 mg depending on the sample.If the slight decrease in resolution is acceptable,much more samples could be applied.Lipoxidase,phoshoglucose isomerase and lactate dehydrogenase could be purified to a great extent with high recovery of activity (more than 80%).