Effectof Bisulfite on Lobster Shell Phenoloxidase

" The role of bisulfite inhibition on the phenoloxidase(PO)catalyzed melanosis reaction in lobster was investigated.Busulfite appears to inhibit melanosis by two mechchanisms:(1)by reacting with intermediate quinones in the melanosis reaction,forming sulfoquinones,and (2)by irreversibly reaching with PO causing complete inactivation.The first mechanism is important because intermediate quinones(dopachrome)are the chroma for spectrophotometric analysis of PO activity."